Abstract: The isotopic fingerprints of uranium are of particular interest for nuclear forensics and nuclear safeguards. Especially the need for lower detection uncertainties of minor isotopes，including ²³⁴U and ²³⁶U, is considered to be of major importance for establishing relations between materials. This work described the utilization of multiple collector inductively coupled plasma mass spectrometry (MC-ICP-MS) for the determination of uranium isotope ratios of uranium materials. The EC nuclear reference material 199 (EC-NRM199) were diluted to about 40.0 ng/g by 2% HNO₃ (V/V). The digested liquid samples of two kinds of yellow cakes were diluted to about 21 000 ng/g and 180 ng/g, respectively. Mass fractionation was corrected by external standardization and standard sample bracketing (SSB) method. The analytical results of the 180 ng/g uranium sample solution showed that the precision for the major isotope ratio ²³⁵U/²³⁸U could be smaller than 0.014%. A faraday-ground-connect method was developed to measure the minor isotope ratios, such as ²³⁴U/²³⁵U, ²³³U/²³⁵U and ²³³U/²³⁴U by faraday cups. The electric current produced by ²³⁸U⁺ in the faraday cup was conducted to the ground potential before arriving the preamplifier. The relative uncertainty for ²³⁴U/²³⁵U could be smaller than 0.020% for the 21 000 ng/g uranium sample solution under magnetscan mode. Variation of the measured isotope signatures would help in identification and source attribution of different uranium materials. An alternative method for analyzing ²³⁶U/²³⁵U was to set the magnet value of the center faraday detector named Ax at mass 234. Through the method, the relative uncertainty of ²³⁶U/²³⁵U for both GBW04234 and GBW04238 was less than 0.11%, and the results agreed with the certificated values within uncertainty range. The results demonstrated that this technique is a precise and accurate method for the determination of uranium isotopic fingerprints in nuclear forensics and nuclear safeguards. Furthermore, the faraday-ground-connect method shows great potential in measuring minor isotope ratios of other elements for nuclear and geological applications.