Abstract: In order to comprehensively understand the chemical composition of Yiqi Jiangzhi Granules, ultra-high performance liquid chromatography-linear ion trap/electro-static orbitrap combined high resolution mass spectrometry (UPLC-LTQ Orbitrap MS) was applied. By comparing the exacted molecular mass, retention time and mass spectrometry fragment ion information of each component to reference substance and related literature, a total of 97 chemical composition were identified, including flavonoids, alkaloids, phenolic acids, anthraquinones, triterpenoids, sesquiterpenoids and other components. Besides, tricin, tricin-7-O-glucoside, 8-acetyl-7-hydroxy-4-methylcoumarin and 3-acetylcoumarin were identified from the ten medicinal ingredients in Yiqi Jiangzhi prescription for the first time. Meanwhile, this study can provide a reference for the research of Yiqi Jiangzhi Granules quality control and pharmacodynamics development.