Abstract: Diaphragma Juglandis Fructus, the dry wood diaphragm tissue that grows inside the Juglans regia L., has been used for treating many diseases such as kidney asthenia, chronic glomerulonephritis and chronic renal insufficiency. Analytical methods for determination of trace elements in Diaphragma Juglandis Fructus have been reported. The main pretreatment method is wet digestion, which has some shortcomings such as tedious preparation steps, long digestion time and high chemical reagent consumption. Therefore, it is essential to develop an appropriate analytical methodologies for determination of trace elements in Diaphragma Juglandis Fructus. In this work, a method of semidigestioninductively coupled plasma mass spectrometry (ICPMS) was developed for the determination of manganese, copper, strontium and barium trace elements in Diaphragma Juglandis Fructus. In order to get good digestion efficiencies of the target elements, the important factors affecting the semidigestion procedure were discussed, such as nature of acid solution, volume of concentrated nitric acid and digestion time. Finally, 0.2 g Diaphragma Juglandis Fructus sample treated with 2 mL of concentrated nitric acid and then transferred to graphite digestion instrument(120 ℃) for 10 min. Then the digestion solution was filtered by 045 μm polyethersulfone filtration membrane and detected by ICPMS. Results of semidigestion method were compared with those obtained with microwaveassisted digestion as reference method. The results showed that the standard curve of each element has a good linear correlation with a coefficient higher than 0999 8. The detection limits are 0037011 μg/L. The relative standard deviations (RSDs) and average recoveries are in the range of 045%299% and 957%1093%, respectively. The results of national standard substance (GBW 10020 citrus leaf) are all within the standard reference value range. The results of ttest indicated that difference in the results from semidigestion and microwaveassisted digestion is not significant. The method is simple, rapid and accurate, which is suitable for determination of the 4 trace elements in Diaphragma Juglandis Fructus.