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基于UPLC-Q-Exactive Orbitrap-MS分析藏药诃子与毛诃子化学成分

周坤, 简平, 梁文仪, 梁林金, 叶婷, 常子豪, 张秋楠, 张兰珍

周坤, 简平, 梁文仪, 梁林金, 叶婷, 常子豪, 张秋楠, 张兰珍. 基于UPLC-Q-Exactive Orbitrap-MS分析藏药诃子与毛诃子化学成分[J]. 质谱学报, 2020, 41(3): 254-267. DOI: 10.7538/zpxb.2019.0070
引用本文: 周坤, 简平, 梁文仪, 梁林金, 叶婷, 常子豪, 张秋楠, 张兰珍. 基于UPLC-Q-Exactive Orbitrap-MS分析藏药诃子与毛诃子化学成分[J]. 质谱学报, 2020, 41(3): 254-267. DOI: 10.7538/zpxb.2019.0070
shu
ZHOU Kun, JIAN Ping, LIANG Wen-yi, LIANG Lin-jin, YE Ting, CHANG Zi-hao, ZHANG Qiu-nan, ZHANG Lan-zhen. Analysis on Chemical Constituents from Terminalia chebula Retz. and Terminalia bellerica (Gaertn.) Roxb. by UPLC-Q-Exactive Quadrupole-Orbitrap Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2020, 41(3): 254-267. DOI: 10.7538/zpxb.2019.0070
Citation: ZHOU Kun, JIAN Ping, LIANG Wen-yi, LIANG Lin-jin, YE Ting, CHANG Zi-hao, ZHANG Qiu-nan, ZHANG Lan-zhen. Analysis on Chemical Constituents from Terminalia chebula Retz. and Terminalia bellerica (Gaertn.) Roxb. by UPLC-Q-Exactive Quadrupole-Orbitrap Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2020, 41(3): 254-267. DOI: 10.7538/zpxb.2019.0070
shu
周坤, 简平, 梁文仪, 梁林金, 叶婷, 常子豪, 张秋楠, 张兰珍. 基于UPLC-Q-Exactive Orbitrap-MS分析藏药诃子与毛诃子化学成分[J]. 质谱学报, 2020, 41(3): 254-267. CSTR: 32365.14.zpxb.2019.0070
引用本文: 周坤, 简平, 梁文仪, 梁林金, 叶婷, 常子豪, 张秋楠, 张兰珍. 基于UPLC-Q-Exactive Orbitrap-MS分析藏药诃子与毛诃子化学成分[J]. 质谱学报, 2020, 41(3): 254-267. CSTR: 32365.14.zpxb.2019.0070
shu
ZHOU Kun, JIAN Ping, LIANG Wen-yi, LIANG Lin-jin, YE Ting, CHANG Zi-hao, ZHANG Qiu-nan, ZHANG Lan-zhen. Analysis on Chemical Constituents from Terminalia chebula Retz. and Terminalia bellerica (Gaertn.) Roxb. by UPLC-Q-Exactive Quadrupole-Orbitrap Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2020, 41(3): 254-267. CSTR: 32365.14.zpxb.2019.0070
Citation: ZHOU Kun, JIAN Ping, LIANG Wen-yi, LIANG Lin-jin, YE Ting, CHANG Zi-hao, ZHANG Qiu-nan, ZHANG Lan-zhen. Analysis on Chemical Constituents from Terminalia chebula Retz. and Terminalia bellerica (Gaertn.) Roxb. by UPLC-Q-Exactive Quadrupole-Orbitrap Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2020, 41(3): 254-267. CSTR: 32365.14.zpxb.2019.0070
shu

基于UPLC-Q-Exactive Orbitrap-MS分析藏药诃子与毛诃子化学成分

  • 北京中医药大学,北京102488

Analysis on Chemical Constituents from Terminalia chebula Retz. and Terminalia bellerica (Gaertn.) Roxb. by UPLC-Q-Exactive Quadrupole-Orbitrap Mass Spectrometry

  • Beijing University of Chinese Medicine, Beijing 102488, China

摘要

    摘要
  • 摘要: 采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用技术（UPLC-Q-Exactive Orbitrap-MS），在电喷雾负离子模式下对使君子科(Combretaceae)榄仁树属(Terminalia Linn.)成分相近的藏药诃子与毛诃子的化学成分进行快速识别和鉴定。采用Acquity UPLC HSS T3色谱柱(21 m×100 mm×18 μm)，以含01%乙酸的甲醇01%乙酸水溶液为流动相进行梯度洗脱。通过高分辨质谱给出的分子离子峰和碎片离子数据，结合相关文献和对照品，从诃子中鉴定出94个成分，包括28个特有成分，从毛诃子中鉴定出96个成分，包括30个特有成分，二者共有成分66个，其中12个鞣质类成分为首次从榄仁树属内发现。本研究可为诃子与毛诃子的快速鉴定，质量控制及阐明其药效基础提供参考。
    Abstract: A highly sensitive and selective method of ultra-performance liquid chromatography coupled with hybrid quadrupole-Orbitrap mass spectrometry (UPLCQExactive OrbitrapMS) was developed to rapidly identify the chemical constituents from Terminalia chebula Retz. and Terminalia bellerica (Gaertn.) Roxb. The separation of the compounds was carried out on an Acquity UPLC HSS T3 column (21 m×100 mm×18 μm). The mobile phase of 01% acetic acid in methanol and 01% acetic acid in water was delivered at a flow rate of 03 mL/min under a gradient program. Mass spectrometer was operated in negative electrospray ionization mode and mass spectra were recorded by scanning the mass range of m/z 1001 500 in both MS and MS/MS modes. Based on retention time, exact mass, molecular formula, fragmentation patterns and references, 94 compounds were identified in T. chebula and 96 compounds were identified in T. bellerica, of which 12 compounds were detected and characterized for the first time in the Terminalia Linn genus. Among them, 66 compounds were the same, 28 specific compounds belonged to T. chebula, 30 specific compounds belonged to T. bellerica. According to references, the differential compounds possessed various biological activities, including antioxidant, antibacterial, antiviral, antimalarial, hepatoprotective and anticancer effects. The UPLCQExactive OrbitrapMS platform was a powerful tool for the determination of chemical constituents and provided useful information to further distinction of T. chebula and T. bellerica. The aim of this study was the comprehensive characterization of compounds in two Terminalia species to providing the reference for rapidly identifying, establishing possible structureactivity relationships and guiding rational drug use in clinical. Certainly, the main limitation of this work was the lack of further purification, identification and confirming the validity of the differential compounds, which should be carried out in further experimental validation.
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