Abstract: The seed of Rosa roxburghii is the seed of the roxburgh rose fruit, which has obvious antioxidant, anti-inflammatory, hypoglycemic, hepatoprotective and anticancer functions. And the seed of R. roxburghii has outstanding biological activity, but the active ingredients are unclear. Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS^E) was widely used for separation and identification of chemical components from traditional Chinese medicine. The UNIFI screening platform can automatically match the theoretical fragment and mass fragment information of the compound in the database, and obtain the identification results. Therefore, UPLC-Q-TOF-MS^E technology combined with UNIFI screening platform was used to analyze and identify the chemical components of the roxburgh rose seed. The chromatographic separation was performed on a Acquity BEH C18 column (100 mm×2.1 mm×1.7 μm). Gradient elution was carried out with 0.1% formic acid solution (A) and acetonitrile (B) as the mobile phase. The column temperature was 40 ℃, the sample chamber temperature was 10 ℃, the flow rate was 0.3 mL/min, and the injection volume was 2 μL. The UNIFI screening platform automatically matched the theoretical fragment and mass fragment information of the compound in the database, and the identification results were obtained. Based on the accuracy of the relative molecular mass, fragment ions, retention behavior, and compared to the database, a total of 55 components were putatively identified, including 19 flavonoids, 36 triterpenes. This established method lays a foundation for further study of seed of R. roxburghii.