Abstract: The information of the trace elements in egg shells surface is of great significance for identifying the type of eggs and judging the quality of the eggs. In this paper, an analytical method for sequential analysis of trace elements in egg shells was developed by coupling on-line extraction with ICP-MS analysis. This method employed ultrapure water and 5% dilute nitric acid as eluents, respectively, to extract water-soluble trace elements fraction and acid-soluble trace elements fraction in egg shells sequentially, followed by transferred online to ICP-MS. By using this method, 15 kinds of trace elements (Na, K, Mg, Cr, Mn, Fe, Zn, Cu, Pb, Al, Cd, As, Sn, Ni, Ti) with water soluble components and acid-soluble components can be obtained in a single run within 10 min, which has the advantages of minimal sample pretreatment, speediness, and low sample consumption (1 mm×1 mm). 15 kinds of trace elements on the egg shells of Ganzhou native eggs, Ganzhou nutritious eggs, Ganzhou grass eggs, Maoancun Maoshi native eggs, Taihe native eggs were investigated. The results showed that 15 kind of trace elements had significant difference (p<0.001) in content occurrence and distribution among different types of eggs. For example, in Ganzhou native eggs, Na was the the most abundant element, followed by Mg, Ti, K, Cr, Pb, Fe, Zn, Ni, Cu, while Mn, Al, As, Cd, Sn were barely detected. Acid-soluble fraction was the dominant fraction for most of elements. Na, K, Pb were most distributed in the cusp of egg, Mg and Ti were most distributed in the middle of egg, while the distribution of Cr and Fe was almost the same in cusp, middle and big head of egg. Compared with the five type of eggs, it can be found that the element content in Ganzhou grass eggs was significantly higher than that in the other type eggs. Interestingly, the information of trace elements content, fraction and distribution on the egg shell can constitute a "fingerprint" for egg. The trace elements "fingerprint" of different types of eggs are significantly different, which was probably related to the fodder and water fed for chook, and the sport of chook. This "fingerprint" have promising application in identifying the type of egg and determining the quality of the egg.