Abstract: This paper reviewed the development of chromatography-mass spectrometry about promoting the analysis of multi-pesticide residues from the selectivity of low resolution mass spectrometry. The progress of mass spectrometry technology had changed the analytical method of pesticide residues. On the basis of clarifying the molecular structure of sample disruptors, the corresponding sample pretreatment method was established. The selective detector of chromatography was replaced by mass spectrometry detector which collected molecular structure information. Selective ion detection technology and tandem mass spectrometry technology improved the ability of eliminate interferents. Based on mass spectrometry, methods for simultaneous multi-residue analysis of multiple pesticides were established.