Abstract: Combining the characteristics of iodine, the research progress of iodine mass spectrometry methods was reviewed. In recent years, the development of inductively coupled plasma mass spectrometry (ICP-MS) technology has focused on coping with more complex samples and solving interference problems in the measurement process. At the same time, the technology of coupling with the sampling and separation system is also continuously developing. And accelerator mass spectrometry (AMS) is moving towards more miniaturization and higher sensitivity. Before ICP-MS measurement, the sample needs to be pre-treated to digest and extract iodine. The sample preparation methods mainly include alkaline extraction, acid digestion, thermal hydrolysis, dry ashing, combustion method and integrated means combined with microwave assistance. In addition to digestion and iodine extraction of the sample before AMS measurement, further separation, enrichment and purification are required. The main methods include solvent extraction, ion exchange chromatography, AgI direct precipitation, AgI-AgCl co-precipitation and so on. This paper summarized the development of ICP-MS and AMS and their applications in iodine measurement, and sorted out the pretreatment methods of iodine-containing samples used for ICP-MS and AMS measurement. Finally, the latest developments in mass spectrometry methods for total iodine, iodine isotopes and speciation of iodine at home and abroad were reviewed.