Abstract: To investigate the volatile components from the rhizomes of Polygonum cuspidatum and its anti-migration activity, the rhizomes of Polygonum cuspidatum was extracted with supercritical fluid carbon dioxide extraction (SFE-CO₂) and analyzed by gas chromatography-mass spectrometry (GC/MS). The would healing model was used to evaluate the effects of the supercritical extracts on human hepatoma Huh-7 cell migration. Thirty-three compounds are identified from the SFE-CO₂ extracts of the rhizomes of Polygonum cuspidatum, the major constituents are curzerene (41.8%), propylene glycol (12.2%), acetic acid (6.9%), germacrene B (6.5%) and 2,3-butanediol (5.9%) ect. The in-vitro would healing model assay shows the volatile constituents of the rhizomes of Polygonum cuspidatum possess effective anti-migration activity against human hepatoma Huh-7 cells by 19.85%. The results will provide foundations for understanding the constituents and further exploritatin of Polygonum cuspidatum.