Abstract: Hydrogen isotope of water in quartz inclusion was measured by sealed quartz tube method. The quartz inclusion sample was first loaded into a quartz tube and then evacuated and sealed. The water was purifed and moved to another quartz tube which contained Cr powder. The quartz tube was evacuated and sealed. This sealed quartz tube was put in muffle furnace at high temperature to produce hydrogen gas for hydrogen isotope analysis. The measured δD value for IAEA-CH₇ is （-100.4±1.0）‰(n=20), displaying a high reproducibility. Meanwhile, two quartz inclusion samples were analyzed, which have δD values of （-66.1±1.0）‰（n=6） and （-74.7±1.0）‰（n=6）, respectively. The result is good enough and demonstrate the reliability of this method. The sealed quartz tube method has a large sample throughput, time saving and avoid contamination among samples as occurred in the conventional method.