Abstract: In recent years, oil spill and its impact on marine environment occurs frequently. To solve this problem, study of oil degrading was put on the agenda. A kind of pseudomonas aeruginosa named SJTD-1 was isolated from soil contaminated by crude oil. SDS-PAGE-1DLC-MS and offline-2DLC-MS methods were used to analysis proteome of SJTD-1 respectively. 1453 proteins with SDS-PAGE-1DLC-MS and 1 623 protenis with offline-2DLC-MS were identified， respectively， and the total identified proteins were 1912 by the two methods. Comparing the two different techniques, offline-2DLC-MS method is more benefit to identify membrane proteins, proteins with molecular weight larger than 30 ku and proteins with pI value less than or equal with eight. GO functional annotation, cellular component, functional enrichment and clustering were used to analysis the total proteins. The proteome analysis of pseudomonas aeruginosa SJTD-1 would promote the application on marine environment bioremediation by SJTD-1.