基于UPLC-Q-TOF MS的吴茱萸致肝毒性部位及入血成分分析

李文兰, 孙向明, 陈晨, 刘悦, 宋辉, 丁晶鑫, 徐蓓蕾, 阎新佳

李文兰, 孙向明, 陈晨, 刘悦, 宋辉, 丁晶鑫, 徐蓓蕾, 阎新佳. 基于UPLC-Q-TOF MS的吴茱萸致肝毒性部位及入血成分分析[J]. 质谱学报, 2017, 38(3): 282-293. DOI: 10.7538/zpxb.youxian.2016.0065
引用本文: 李文兰, 孙向明, 陈晨, 刘悦, 宋辉, 丁晶鑫, 徐蓓蕾, 阎新佳. 基于UPLC-Q-TOF MS的吴茱萸致肝毒性部位及入血成分分析[J]. 质谱学报, 2017, 38(3): 282-293. DOI: 10.7538/zpxb.youxian.2016.0065
LI Wen-lan, SUN Xiang-ming, CHEN Chen, LIU Yue, SONG Hui, DING Jing-xin, XU Bei-lei, YAN Xin-jia. Analysis of Euodia rutaecarpa Hepatotoxic Part and Absorbed Components in Rat Serum Based on UPLC-Q-TOF MS[J]. Journal of Chinese Mass Spectrometry Society, 2017, 38(3): 282-293. DOI: 10.7538/zpxb.youxian.2016.0065
Citation: LI Wen-lan, SUN Xiang-ming, CHEN Chen, LIU Yue, SONG Hui, DING Jing-xin, XU Bei-lei, YAN Xin-jia. Analysis of Euodia rutaecarpa Hepatotoxic Part and Absorbed Components in Rat Serum Based on UPLC-Q-TOF MS[J]. Journal of Chinese Mass Spectrometry Society, 2017, 38(3): 282-293. DOI: 10.7538/zpxb.youxian.2016.0065
李文兰, 孙向明, 陈晨, 刘悦, 宋辉, 丁晶鑫, 徐蓓蕾, 阎新佳. 基于UPLC-Q-TOF MS的吴茱萸致肝毒性部位及入血成分分析[J]. 质谱学报, 2017, 38(3): 282-293. CSTR: 32365.14.zpxb.youxian.2016.0065
引用本文: 李文兰, 孙向明, 陈晨, 刘悦, 宋辉, 丁晶鑫, 徐蓓蕾, 阎新佳. 基于UPLC-Q-TOF MS的吴茱萸致肝毒性部位及入血成分分析[J]. 质谱学报, 2017, 38(3): 282-293. CSTR: 32365.14.zpxb.youxian.2016.0065
LI Wen-lan, SUN Xiang-ming, CHEN Chen, LIU Yue, SONG Hui, DING Jing-xin, XU Bei-lei, YAN Xin-jia. Analysis of Euodia rutaecarpa Hepatotoxic Part and Absorbed Components in Rat Serum Based on UPLC-Q-TOF MS[J]. Journal of Chinese Mass Spectrometry Society, 2017, 38(3): 282-293. CSTR: 32365.14.zpxb.youxian.2016.0065
Citation: LI Wen-lan, SUN Xiang-ming, CHEN Chen, LIU Yue, SONG Hui, DING Jing-xin, XU Bei-lei, YAN Xin-jia. Analysis of Euodia rutaecarpa Hepatotoxic Part and Absorbed Components in Rat Serum Based on UPLC-Q-TOF MS[J]. Journal of Chinese Mass Spectrometry Society, 2017, 38(3): 282-293. CSTR: 32365.14.zpxb.youxian.2016.0065

基于UPLC-Q-TOF MS的吴茱萸致肝毒性部位及入血成分分析

Analysis of Euodia rutaecarpa Hepatotoxic Part and Absorbed Components in Rat Serum Based on UPLC-Q-TOF MS

  • 摘要: 利用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF MS)法分析鉴定吴茱萸肝毒性部位的化学成分和给大鼠灌胃毒性部位后的血中原形成分及代谢产物。在优化的色谱和质谱条件下,采用正离子扫描模式对质量范围m/z 50~1 200的化合物进行数据采集,分析时间仅为12 min。通过UNIFI软件与人工相结合的方式对采集的数据进行分析,根据高分辨质谱提供的母离子与碎片离子的精确质量信息,初步推测了化合物的分子组成;结合标准品和参考文献数据,共鉴定了肝毒性部位中的29个化学成分,其中包括9个吲哚类生物碱、10个喹诺酮类生物碱、5个三萜、3个黄酮、1个其他类生物碱和1个有机酸。进一步比较了体内外样品的基峰离子色谱图,结合质谱数据和化合物裂解规律,共鉴定出21个入血成分,包括15个原形成分和6个代谢产物(1个为未知成分),并推测了代谢产物的代谢途径。该方法可为揭示吴茱萸肝毒性成分提供可靠的数据支持。
    Abstract: A method of ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) was established to analyze the chemicals in Euodia rutaecarpa hepatotoxic part and metabolic components in rat blood. Under the optimized condition of chromatography and mass spectrometry, the data collection for the compounds in the mass range of m/z 50-1200 was performed using positive ion scanning mode within 12 min. The collected data were analyzed based on UNIFI software combined with artificial analysis. According to the precise mass information of precursor ions and fragment ions offered by high resolution mass spectrometry, the molecule composition of compound was preliminarily speculated. Finally, a total of 29 constituents in hepatotoxic part are identified combined with substances and reference data, including 9 indole alkaloids, 10 quinolone alkaloids, 5 triterpenoids, 3 flavonoids, one other alkaloid and one organic acid. 21 compounds in blood are characterized, including 15 prototypes and 6 metabolites (a compound unknown) by comparing the base peak ion chromatograms of samples in vivo and in vitro combined with MS data, compound fragmentation laws and published literatures. The metabolic pathways of metabolites were further speculated. This method can provide, a reliable data support to indicate hepatotoxic components of Euodia rutaecarpa.
  •   2588

  • [1] 中国药典委员会. 中华人民共和国药典(一部)[M]. 北京:化学工业出版社,2010.
    [2] SUGIMOTO T, MIYASE T, KUROYANAGI M, et al. Limonoids and quinolone alkaloids from Evodia rutaecarpa Bentham[J]. Chemical and Pharmaceutical Bulletin, 1988, 36(11): 4453-4461.
    [3] TANG Y Q, FENG X Z, HUANG L. Quinolone alkaloids from Evodia rutaecarpa[J]. Phytochemistry, 1996, 43(3): 719-722.
    [4] TANG Y Q, FENG X Z, HUANG L. Studies on the chemical constituents of Evodia rutaecarpa Benth[J]. Journal of Chinese Pharmaceutical Sciences, 1996, 31(2): 151-155.
    [5] CAI G X, HUANG D, LI S X, et al. Comparative analysis of essential oil components of Evodia rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang and Evodia rutaecarpa (Juss.) Benth[J]. Natural Product Research, 2012, 26(19): 1796-1798.
    [6] XU Y, LIU Q, XU Y, et al. Rutaecarpine suppresses atherosclerosis in ApoE-/- mice through upregulating ABCA1 and SR-BI within RCT[J]. Journal of Lipid Research, 2014, 55(8): 1634-1647.
    [7] WEN Z, FENG S, WEI L, et al. Evodiamine, a novel inhibitor of the Wnt pathway, inhibits the self-renewal of gastric cancer stem cells[J]. International Journal of Molecular Medicine, 2015, 36(6): 657-663.
    [8] ZHAO N, LI Z L, LI D H. Quinolone and indole alkaloids from the fruits of Euodia rutaecarpa and their cytotoxicity against two human cancer cell lines[J]. Phytochemistry, 2015, 109: 133-139.
    [9] 蔡雪映,孟楠,杨冰. 服用吴茱萸过量致中毒1例分析[J]. 北京中医药,2006, 25(3): 171-172.CAI Xueying, MENG Nan, YANG Bing. Analysis of one poisoning case caused by excessive Evodiae fructus[J]. Beijing Journal of TCM, 2006, 25(3): 171-172(in Chinese).
    [10] COHEN S M, HEYWOOD E. Hepatotoxicity associated with the use of white flood, a nutritional supplement[J]. Practical Gastroenterology, 2012, 10: 45-48.
    [11] TESCHKE R, WOLFF A, FRENZEL C, et al. Review article: herbal hepatotoxicity-an update on traditional Chinese medicine preparations[J]. Aliment Pharmacol Ther, 2014, 40(1): 32-50.
    [12] 周绮,张茜,金若敏. 吴茱萸致小鼠肝毒性时效、量效关系研究[J]. 中国实验方剂学杂志,2011,17(9):232-235.ZHOU Qi, ZHANG Qian, JIN Ruomin. Time-effect and dose-effect of Evodia rutaecarpa on hepatotoxicity in mice[J]. Chin J Exp Tradit Med Formulae, 2011, 17(9): 232-235(in Chinese).
    [13] 黄伟,李晓骄阳,孙蓉. 吴茱萸水提组分多次给药对小鼠肝毒性的“量-时-毒”关系研究[J]. 中国中药杂志,2012, 37(15): 2223-2227.HUANG Wei, LI Xiaojiaoyang, SUN Rong. “Dose-time-toxicity” relationship study on hepatotoxicity caused by multiple dose water extraction components of Evodiae fructus to mice[J]. China Journal of Chinese Materia Medica, 2012, 37(15): 2223-2227(in Chinese).
    [14] 李晓宇,吴晓文,窦立雯,等. 吴茱萸挥发油多次给药致小鼠肝毒性氧化损伤机制研究[J]. 中国药物警戒,2015,12(1):16-19.LI Xiaoyu, WU Xiaowen, DOU Liwen, et al. Research of oxidative damage mechanism on hepatotoxicity caused by volatile oil from Evodia fructus in mice[J]. Chinese Journal of Pharmacovigilance, 2015, 12(1): 16-19(in Chinese).
    [15] CAI Q, WEI J, ZHAO W, et al. Toxicity of Evodiae fructus on rat liver mitochondria: the role of oxidative stress and mitochondrial permeability transition[J]. Molecules, 2014, 19(12): 21168-21182.
    [16] 廖文强,李波,李莉,等. 吴茱萸致小鼠肝毒性分子机制研究[J]. 中国中药杂志,2014,39(24):4865-4868.LIAO Wenqiang, LI Bo, LI Li, et al. Study on molecular mechanism of Euodiae fructus on liver toxicity in mice[J]. China Journal of Chinese Materia Medica, 2014, 39(24): 4865-4868(in Chinese).
    [17] CHEN Y, MONSHOUWER M, FITCH W L. Analytical tools and approaches for metabolite identification in early drug discovery[J]. Pharmaceutical Research, 2007, 24(2): 248-257.
    [18] CASTRO-PEREZ J M. Current and future trends in the application of HPLC-MS to metabolite identification studies[J]. Drug Discovery Today, 2007, 12(5/6): 249-256.
    [19] SHOJI N, UMEYAMA A, IUCHI A, et al. Two novel alkaloids from Evodia rutaecarpa[J]. Journal of Natural Products, 1989, 52(5):1160-1162.
    [20] 邱时祎. 吴茱萸中生物碱的分离纯化和质谱表征[D]. 上海:华东理工大学,2013.
    [21] 高鹏,王灵芝,邬瑞光,等. 吴茱萸碱和吴茱萸次碱电喷雾-离子阱质谱裂解途径研究[J]. 药物分析杂志,2012,32(5):772-774.GAO Peng, WANG Lingzhi, WU Ruiguang, et al. ESI-ion-trap MS study on fragmentation pathways of evodiamine and rutecarpine[J]. Chinese Journal of Pharmaceutical Analysis, 2012, 32(5): 772-774(in Chinese).
    [22] 马微,马强,朱明达,等. 超高效液相色谱-串联质谱测定减肥保健食品中辛弗林及其电喷雾质谱裂解途径研究[J]. 分析科学学报,2010,26(6):636-640.MA Wei, MA Qiang, ZHU Mingda, et al. ESI fragmentation study and determination of synephrine in the weight-loss functional foods by ultra performance liquid chromatography-tandem mass spectrometry[J]. Journal of Analytical Science, 2010, 26(6): 636-640(in Chinese).
    [23] 廖琼峰,谢智勇,张蕾,等. 吴茱萸中吲哚喹唑啉类生物碱和吴茱萸内酯的质谱裂解分析[J]. 中药材,2008,31(5):673-676.LIAO Qiongfeng, XIE Zhiyong, ZHANG Lei, et al. Mass spectrometric analysis of quinazoline alkaloid and limonin from Fructus Evodiae[J]. Journal of Chinese Medicinal Materials, 2008, 31(5): 673-676(in Chinese).
    [24] 李丽. 吴茱萸碱、吴茱萸次碱和左旋四氢巴马亭在微生物和大鼠体内的代谢研究[D]. 沈阳:沈阳药科大学,2005.
    [25] SUN H Z, FANG Z Z, CAO Y F, et al. Investigation of the in vitro metabolism of evodiamine characterization of metabolites and involved cytochrome p450 isoforms[J]. Phytotherapy Research, 2013, 27(5): 705-712.
    [26] UENG Y F, DON M J, JAN W C, et al. Oxidative metabolism of the alkaloid rutaecarpine by human cytochrome P450[J]. Drug Metabolism and Disposition the Biological Fate of Chemicals, 2006, 34(5): 812-827.
    [27] LIN L C, LI S H, WU Y T, et al. Pharmacokinetics and urine metabolite identification of dehydroevodiamine in the rat[J]. Journal of Agricultural and Food Chemistry, 2012, 60(7): 1595-1604.
图(1)
计量
  • 文章访问数:  983
  • HTML全文浏览量:  1
  • PDF下载量:  829
  • 被引次数: 0
出版历程
  • 刊出日期:  2017-05-19

目录

    /

    返回文章
    返回