Abstract: The bifonazole's plasma concentration in rabbits after bifonazole solution preparation and cream preparation spread on rabbit skin was determined,using GC/MS-SIM quantitative method,and their pharmacokinetic parameters were calculated with 3p87 software.The results showed that the plasma concentration-time curves of the two preparations in rabbits were fitted to the two-compartment open model.Tmax of solution and cream preparations were 2.84 h and 5.84 h,and Cmax,356.18ng/ml and 182.81ng/ml,respectively.The concentration of bifonazole in drug-spread skin was quantified:4200ng after one day,2500ng after two days,and 1250ng after three days respectively.However,the concentrations of bifonazole in liver,kidney,muscle,muscle beneath skin and skin for control are lower than the determination limit.