肽质量指纹谱技术的建立及其在人肺癌细胞蛋白质组研究中的初步应用

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DEVELOPMENT AND PRELIMINARY APPLICATION OF PEPTIDE MASS FINGERPRINTING TECHNIQUE IN PROTEOME RESEARCH

Abstract: Proteome means the whole proteins expressed by genome in a cell or tissue.Two- dimensional electrophoresis (2-DE), computer image analysis and mass spectrometry were known as the three key techniques in proteome research. Peptide mass fingerprinting (PMF) is becoming a widely used and rapidly developed technique for protein identification after 2- DE. In this study, a systematic method to identify the proteins after 2-DE by PMF was developed. Proteins were digested in-gel by trypsin and the masses of peptides were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI- TOFMS). the data obtained from PMF were used for protein database searching and protein identification. the proteins from human lung cancer cell isolated by 2-DE were identified by The method developed in this research.

1 Wilkins M. R., Williams K. L