Abstract: This paper was focused on studying the profile of endogenous androgens in human urine of three volunteers,who have higher T/E ratio than normal. The results showed that the concentration ratios of some endogenous androgens, which were monitered by GC/MS-SIM,were more stable both in a day and between days in one month. The three volunteers have physiological higher T/E ratios and normal profile of endogenous androgens in urine