Abstract: Volatile oils were extracted from leaf twigs in Biota orientalis by cellulase extraction (CE) method, simultaneous distillation extraction(SDE) method and stream distillation-extraction method (DSE), the components were identified by gas chromatography-mass spectrometry (GC/MS). The results indicated that the compounds from the volatile oils extracted by the three methods were different. Thirty-two chemical components were identified by CE, the major component of them was cedrene accounting for 41.25% of total volatile components; twenty-nine chemical components were identified by SDE, the major component was cedrol accounting for 39.06%; thirty-two chemical components were identified by DSE, the major component was cedrol accounting for 31.43%, respectively.