高效液相色谱-离子阱质谱联用测定食品中7种苏丹红类染料

Determination of 7 Kinds of Sudan Dye in Food by HPLC-ESI-MS/MS

摘要: 本文研究了高效液相色谱-电喷雾离子阱质谱联用仪准确测定辣椒类食品中7种苏丹红类染料的分析方法。样品中的苏丹红类染料用有机溶剂乙腈提取，直接定容后进行色谱分析。质谱检测采用正离子电离方式。方法的线性范围为5.0~30 μg/L，重现性RSD<5%，回收率为63.5%～106.8%。结果表明本方法具有操作简便、快速、准确等优点。

Abstract: A method for determination of 7 kinds of Sudan dye in food by reversed phase(RP) liquid chromatography-tandem mass spectrometry coupled with electro spray (LC-ESI-MS/MS) was developed. The sample was extracted by acetonitrile and analysed by LC-ESI-MS/MS. The linear range was 5.0~30 μg/L. The average recoveries were 63.5%~106.8% and its relative standard deviation was lower than 5%. The method was sensitive, reliable, selective and reproducible.