阿胶中特异性多肽的分离与液质联用分析

详细信息

Separation of the Unique Peptides from Colla Corri Asini and Sequence Determination with High Performance Liquid ChromatographyMass Spectrometry

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China

Abstract: Colla corri asini, as traditional Chinese medicine “Ejiao”, is a kind of donkeyskin gelatin. The main components in Colla corri asini include proteins, peptides， polysaccharides and amino acids. In this study, the Ejiao solution was fractionated by fibronecti sepharose affinity chromatography. After further demineralization and condensation by solid-phase extraction, some unique peptides were obtained. The peptides obtained were separated with high performance liquid chromatography and their sequences were determined with electrospray ionization mass spectrometry.

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