Abstract: Colla corri asini, as traditional Chinese medicine “Ejiao”, is a kind of donkeyskin gelatin. The main components in Colla corri asini include proteins, peptides， polysaccharides and amino acids. In this study, the Ejiao solution was fractionated by fibronecti sepharose affinity chromatography. After further demineralization and condensation by solid-phase extraction, some unique peptides were obtained. The peptides obtained were separated with high performance liquid chromatography and their sequences were determined with electrospray ionization mass spectrometry.