Abstract: The transfer of amino acids in a key species “Chlorella sp.Calanus sinicus Engraulis japonicus” food chain was studied. It makes for understanding profoundly mechanism of substance and energy flow in marine food webs. Concentrations of amino acids in samples were analyzed by GC/MS after derivatization. Calanus sinicus plays a linchpin function in amino acid enrichment of this food chain and is suitable to growth of Engraulis japonicus rather than synthesized bait despite of the latter has higher amino acid concentrations.