Abstract: The headspace constituents released from fresh flowers of Gardenia were sampled by solid-phase microextraction(SPME). The chemical constituents were separated and identified by gas chromatographymass spectrometry.cis-Ocimene（37.68%），linalool（26.09%），(Z,E)- α-farnesene（12.54%），cyclohexane, chloro（7.38%），benzoic acid, methyl ester（3.92%），cyclobutane carboxylic acid, isopropyl ester（2.77%），spiro4，5decan-1-one, 6-hydroxy（1.54%）were the most abundant volatiles released from fresh flowers of Gardenia , and a total of 70 volatile compounds were identified in this study.