Abstract: Isotopes can be applied to food geographical origin traceability as “fingerprint”. The radioactive disintegration of Sr in different samples leads to variation of ⁸⁷Sr /⁸⁶Sr in nature. Sr in wine comes from soils, rocks and water in vine yard, ⁸⁷Sr /⁸⁶Sr can give much information about the geographical origin of wine. This paper describes in details the separation procedure by Sr-resin and determination by MC-ICP-MS.