Abstract: The contents of lead, iron, copper in red wine were determined by using ICP-MS, ICP-OES, and an authoritative measurement method-isotope dilution mass spectrometry (IDMS). The results indicated that it was not easy to achieve agreements with IDMS by using common methods. If using some methods, such as matrix mathing and adding standards to reduce the inference of matrix, the results could be better. So it is necessary to use similar certified reference material to calibrate the method and correct the results when the complex matrix samples were measured.