Abstract: Since Barber and coworkers developed the method of Fast Atom Bombardment Mass Spectronetry(FABMS) in the early of 1980's,FABMS has become the most powerful mass spectrometric method for the analysis of polar,involatile and thermo-labile compounds. Considering the usefulness of high resolution FABMS in the evaluation of molecular structures,studies on accurate mass measurement by HRFABMS,and other applications of it have not been carried out extensively,in the present paper the experimental methods of HRFABMS,and the applications of HRFABMS in 4 aspects (small biomolecule, large biomolecule,polar products of drug metabolism and clinical pathological analysis) in the field of biomedical analysis were briefly described.