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摘要: 建立了动物源食品中大观霉素,链霉素,双氢链霉素,阿米卡星,卡那霉素,安普霉素,庆大霉素和新霉素等8种氨基糖苷类药物残留检测的超高效液相色谱串联质谱(UPLC-MS/MS)方法。结果表明:8种氨基糖苷类药物在0.05~2 mg•L-1浓度范围内呈现良好的线性关系,相关系数R2均大于0.998;方法检测限为5 μg•kg-1,定量限为10 μg•kg-1;从100、200和300 μg•kg-1 3个添加浓度检测结果可以看出,方法平均回收率为83.0%~114.1%,批内RSD为1.1%~11.4%,批间RSD为2.1%~8.9%,可以满足食品安全有关法规的要求。
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关键词:
- 超高效液相色谱-串联质谱法 /
- 动物源食品 /
- 氨基糖苷类药物
Abstract: Spectinomycin, streptomycin, dihydrostreptomycin, amikacin, kanamycin, apramycin, gentamicin and neomycin in foodstuffs of animal origin were detected by UPLC-MS/MS. The calibration curve is good linear between the peak areas and the concentrations of 0.05~2 mg•L-1,the correlation coefficient R2>0.998. The limit of detection is 5 μg•kg-1, and the limit of quantification is 10 μg•kg-1. The average recoveries from spiked milk at the three concentrations of 100, 200 and 300 μg•kg-1 range from 83.0%~114.1%, and intra- and inter-batch variation coefficients are 1.1%~11.4% and 2.1%~8.9%,respectively.-
Keywords:
- UPLC-MS/MS /
- foodstuffs of animal origin /
- aminoglycosides
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