超高效液相色谱-串联质谱法检测动物源食品中8种氨基糖苷类药物残留

孙雷, 张骊, 黄耀凌, 汪霞, 王树槐

孙雷, 张骊, 黄耀凌, 汪霞, 王树槐. 超高效液相色谱-串联质谱法检测动物源食品中8种氨基糖苷类药物残留[J]. 质谱学报, 2009, 30(1): 60-64.
引用本文: 孙雷, 张骊, 黄耀凌, 汪霞, 王树槐. 超高效液相色谱-串联质谱法检测动物源食品中8种氨基糖苷类药物残留[J]. 质谱学报, 2009, 30(1): 60-64.
SUN Lei, ZHANG Li, HUANG Yao-ling, WANG Xia, WANG Shu-huai. Determination of Aminoglycosides in Foodstuffs of Animal Origin by UPLC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2009, 30(1): 60-64.
Citation: SUN Lei, ZHANG Li, HUANG Yao-ling, WANG Xia, WANG Shu-huai. Determination of Aminoglycosides in Foodstuffs of Animal Origin by UPLC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2009, 30(1): 60-64.

超高效液相色谱-串联质谱法检测动物源食品中8种氨基糖苷类药物残留

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    通讯作者:

    孙雷

  • 中图分类号: O 657.63

Determination of Aminoglycosides in Foodstuffs of Animal Origin by UPLC-MS/MS

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    Corresponding author:

    SUN Lei

  • 摘要: 建立了动物源食品中大观霉素,链霉素,双氢链霉素,阿米卡星,卡那霉素,安普霉素,庆大霉素和新霉素等8种氨基糖苷类药物残留检测的超高效液相色谱串联质谱(UPLC-MS/MS)方法。结果表明:8种氨基糖苷类药物在0.05~2 mg•L-1浓度范围内呈现良好的线性关系,相关系数R2均大于0.998;方法检测限为5 μg•kg-1,定量限为10 μg•kg-1;从100、200和300 μg•kg-1 3个添加浓度检测结果可以看出,方法平均回收率为83.0%~114.1%,批内RSD为1.1%~11.4%,批间RSD为2.1%~8.9%,可以满足食品安全有关法规的要求。
    Abstract: Spectinomycin, streptomycin, dihydrostreptomycin, amikacin, kanamycin, apramycin, gentamicin and neomycin in foodstuffs of animal origin were detected by UPLC-MS/MS. The calibration curve is good linear between the peak areas and the concentrations of 0.05~2 mg•L-1,the correlation coefficient R2>0.998. The limit of detection is 5 μg•kg-1, and the limit of quantification is 10 μg•kg-1. The average recoveries from spiked milk at the three concentrations of 100, 200 and 300 μg•kg-1 range from 83.0%~114.1%, and intra- and inter-batch variation coefficients are 1.1%~11.4% and 2.1%~8.9%,respectively.
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出版历程
  • 收稿日期:  2008-05-25
  • 修回日期:  2008-09-03
  • 刊出日期:  2009-01-19

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