LC/MS和LC-MS/MS定性检测保健品中非法添加的伐地那非

Detection of Vardenafil in Health Products by LC/MS and LC-MS/MS

摘要: 采用LC/MS和LC-MS/MS法同时检测保健品中非法添加的伐地那非。用乙酸乙酯提取，以10 mmol•L^-1的甲酸铵（pH 3.5）和乙腈为流动相，分别用Agilent Zorbax SB C₁₈（150 mm×2.1 mm×5 μm）和Agilent Zorbax SB C₁₈（100 mm×2.1 mm×3.5 μm）色谱柱分离，采用电喷雾离子源，正离子扫描方式进行分析检测。该方法简便、快捷、可靠，适用于保健品中非法添加伐地那非的常规检测。

Abstract: Vardenafil in health products was detected simultaneously by LC/MS and LC-MS/MS. Ethyl acetate was used as the extraction solution. Mobile phase was included 10 mmol•L^-1 ammonium formate (pH 3.5) and acetonitrile. Agilent Zorbax SB C18（150 mm×2.1 mm×5 μm）and Agilent Zorbax SB C18（100 mm × 2.1 mm×3.5 μm）columns were used to separate the ingredients， and mass data were acquired with the ESI(+) mode. The method is simple，rapid and specific. It is suitable for routine detection of vardenafil in health product.