Abstract: In this paper, the study situation of stable isotope standard sample for hydrogen, carbon and oxygen, and the development in analytical technics of organics carbon have been stated generally. From that, conclusions of some important aspect about study on stable isotope of hydrogen and carbon have been drown: First, the variety in composition of coal and kerogen is mainly connected with its type and environment of sedimentation, and has nothing to do with its thermal evolution stage; Second, making oil rock compare by using carbon isotope in chloroform-A, crude oil and its family is a efficent method; The last, using the data of isotope of carbon and hydrogen in natural gas, we can decide the origin-type, maturity of natural gas, and make oil origin compare, and we can decide the origin and cause of the hydrocarbon anomaly of surface geochemical exploration for petroleum by applying the data of carbon isotope as well. However, for composition of carbon isotope could be affected by biological degratation and d stillatic,we must pay attention to it.