Abstract: Reconstituting mass spectra data into virtual image is necessary for imaging mass spectrometry. There is a lack of special wizard style interface software to realize this processing. A software named imgGenerate is developed to guide users to edit parameters according to mass spectra raw data, and step by step to reconstitute them into Analyze 7.5 image format, which can be read by image analysis softwares, such as BioMap and IMSview.ImgGenerate is successfully used to reconstitute mass spectral raw data of rat brain section and writing analyzing into images, this indicates the wizard style interface software is specially useful for reconstituting mass spectra imaging data.