Abstract: The fatty oils of wild Schisanda chinensis bail1 and plant Schisanda chinensis bail1 were extracted by soxhlet method. The fatty acids in the oils were esterified, and analyzed by gas chromatography-mass spectrometry(GC/MS). The results show that 7 fatty acids are detected in wild Schisanda chinensis bail1， and 9 fatty acids are detected in plant Schisanda chinensis bail1， respectively. The major fatty acid is 9,12-octadecadienoic acid in wild Schisanda chinensis bail1 88.56% and in plant Schisanda chinensis bail1 76.64%， respectively. The content of the 9,12-octadecadienoic acids were determinated by HPLC. The column was Kromasil C18, the mobile phase was V(iso-Propyl alcohol)∶V(acetonitrile)= 35∶65, the column temperature was 25 ℃, detection wavelength at 205 nm. The regression equation obtained is y=28 056x - 181.57， r =0.999 9. The method coefficient of variation is smaller than 1.35%, and the recovery of the method is 98.8%－109.1%.