北五味子的超临界萃取及产物的HPLC-APCI-MS/MS分析

CO₂-SFE Extraction of the Lignans in Schisandra chinensis Fruits and Analysis of Extracting Products by HPLC-APCI-MS/MS

摘要: 优化超临界流体萃取北五味子中木脂素类成分的提取工艺，对萃取物中所含有效成分的归属进行分析鉴定。以五味子总木脂素为评价指标，采用正交实验法和不同影响因素系统考察法对CO₂-SFE萃取北五味子中木脂素类成分提取工艺进行优化，利用液相色谱大气压化学电离质谱联用法(HPLC-APCI-MS/MS)分析鉴定产物结构。确定了最佳的超临界提取工艺，即压力为25 MPa，温度为45 ℃，提携剂浓度为90%乙醇，并对萃取产物中所含的10种木脂素类化合物进行归属鉴别。证明优化的工艺与其他常规提取工艺相比较科学合理，具有较强的稳定性和有一定的实用价值。

Abstract: The orthogonal test was used to optimize CO₂-SFE process of the lignans in Schisandra chinensis fruits with content of total lignan as index, and the extraction products were analyzed by HPLC-APCI-MS/MS. The optimal extraction procedure is as follows: the extraction pressure is 25 MPa, the extraction temperature is 45 ℃，and the concentration alcohol is 90%. Ten structures of extraction products are confirmed by HPLC-APCI-MS/MS. The optimum extraction procedure is reasonable and stable. Compared SFE with traditional methods, the optimum extraction procedure is excellent and valuable．