LC-MS/MS和荧光偏振法监测环孢素A血药浓度的相关性研究

Study on Correlation of the Whole Blood Concentrations of Cyclosporine A by LC-MS/MS and TDx

摘要: 建立液相色谱-质谱（LC-MS/MS）监测环孢素A(CsA)血药浓度的方法，并与荧光偏振法（TDx）监测结果比较，进行统计学分析，考察2种方法的相关性。LC-MS/MS方法测定CsA血药浓度平均为（99.9±62.7） μg/L，小于TDx法测定的浓度平均值（199.7±129.1） μg/L，2种监测方法测定结果相关系数r平均为0.929，相关性较好，LC-MS/MS法和TDx法自身配对比值平均为（51.6±9.3）%，因此推测LC-MS/MS法可用于CsA血药浓度监测的治疗窗。LC-MS/MS法测定的浓度为CsA原型药物浓度，能更准确的反映原型药物在体内的准确浓度，更适用于日常血药浓度监测工作。

Abstract: The concentrations of CsA were determined by LC-MS/MS, and which compared with TDx. The correlations of the two method were researched by statisteal analysis. The mean concentration is (99.9±62.7) μg/L by LC-MS/MS, and (199.7±129.1) μg/L by TDx. The correlation of two methods is 0.929, and the two methods have good correlations in statistics. The mean concentration ratio is (51.6±9.3)%. The LC-MS/MS method is a more precise method due to determination the effective concentration of CsA, which is suit for daily TDM.