吹扫捕集-气相色谱/质谱法测定巴山箬竹叶中挥发性成分

Determining Volatile Compounds in Indocalamus bashanensis Leaves by Purge-and-Trap GC/MS

摘要: 为了分离并鉴定大熊猫所食巴山箬竹叶中53种挥发性化合物，本研究将吹扫捕集进样技术与气相色谱质谱法相结合，应用于竹叶中挥发性组分的分析。所鉴定出的物质中，以醇类（18种）、醛类（14种）和酮类（8种）为主，其他化合物为烯烃（6种）、酯类（3种）和杂环类（4种）。在所鉴定物质的气质总离子流图中，峰面积大于4 %的有乙醇（4.78%）、1-戊烯-3-醇（4.90%）、2-乙基呋喃（9.23%）、正己醛（9.07%）、4-己烯-1-醇（28.44%）、1-己醇（4.36%）、2-己烯-1-醇（5.62%）及2-己烯醛（18.28%）。

Abstract: The purge-and-trap extraction method coupled to gas chromatography-mass spectrometry (GC/MS) detection, was applied to the determination of volatile compounds in-Indocalamus bashanensis-leaves. Fifty-three components are identified. There are ketone (8 kinds), aldehyde (14 kinds) and alcohol (18 kinds), and the others are olefins, esters and heterocycles. The identified compounds which peak areas of total ion current (TIC) above 4% are ethyl alcohol (4.78%), 1-penten-3-ol (4.90%), 2-ethyl-furan (9.23%), n-hexanal (9.07%), 4-hexen-1-ol (28.44%), 1-hexanol (4.36%), 2-hexen-1-ol (5.62%) and 2-hexenal (18.28%).