Abstract: The components and the relative contents of the fatty acids in Gleditsia sinensis L am.were analyzed. The Gleditsia sinensis L am.oil was extracted by soxhlet extraction. The fatty acids in the oil were esterified by sodium hydroxide-methanol, and analyzed by gas chromatography-mass spectrometry (GC/MS). 14 fatty acids are identified in the Gleditsia sinensis L am.. The main components are unsaturated fatty acids in the Gleditsia sinensis L am..