Abstract: The chromium species in urine of chromate workers were determined using ion-pair reversed-phase HPLC-ICP-MS hyphenated technique (IPRPC-ICP-MS). Our results showed a significant relationship (n＝32, p＜0.01) between the urinary Cr(VI) and the individual airborne exposure levels of chromate workers, indicating that the urinary Cr(VI) level could be used as a convenient and suitable monitor for acute Cr(VI) occupational exposure. The addition of chelating agent of Cr(VI) (tetraethylammonium, TEA), could significantly enhance the stability of urinary Cr(VI) during sample storage. Meanwhile, the pH values had no significant effects on the Cr(VI) stability when samples were stored at －20℃ or －80℃.