Abstract: The quantitative methods of proteomics include the relative quantitation and absolute quantitation of proteins. Relative quantitation of proteomics is the analysis of the relative changes of the expressed proteins in the samples originated from different physiological and pathological situations. The absolute quantitation of proteomics is to determine the absolute amount or concentration of each protein in a biological sample, such as cells,tissue or body fluid. Currently, the main strategies of absolute quantitation of proteomics are composed of the quantitative methods based on internal standard strategy and the label-free technique with the statistic analysis of MS data. The quantitative information of the proteome can help us understand the biological function of proteins in the interaction network. With the determination of the absolute quantitation of the biomarker, we can directly judge the status of a disease, which will benefit the clinical diagnosis and the treatment of the disease.