刺五加叶的HPLC-UV和ESI-MS指纹图谱研究
The Fingerprints of Leaves of Acanthopanax Senticosus by HPLC-UV and ESI-MS
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摘要: 对12批不同来源的刺五加叶提取物进行指纹图谱研究,并利用ESI-MS指纹图谱鉴别刺五加叶与山楂叶。分别采用高效液相色谱(HPLC-UV)和电喷雾电离质谱(ESI-MS)测定12批不同来源的刺五加叶提取物,利用ESI-MS技术测定刺五加叶与山楂叶提取物,得到了分离度、精密度和重现性均较好的刺五加叶HPLC-UV及ESI-MS指纹图谱;同时,利用刺五加叶与山楂叶ESI-MS指纹图谱的差异,成功鉴别了二者,可为刺五加叶药材的质量控制提供参考。Abstract: The extracts of leaves of Acanthopanax Senticosus were studied by HPLC-UV and ESI-MS. 12 samples from different sources were detected by HPLC-UV and ESI-MS. Perfect fingerprints were obtained. Meanwhile, leaves of Acanthopanax Senticosus and leaves of Folum crataegi were identified successfully by using the fingerprints of ESI-MS. The method is simple, accurate. It can be used for the quality control of leaves of Acanthopanax Senticosus.