LC-MS/MS法定性检测保健品中非法添加的降糖药

Detection of Antidiabetics in Health Products by LC-MS/MS

摘要: 建立了同时检测保健品中非法添加的10种化学降糖药的分析方法。用甲醇直接提取，以10 mmol•L^-1 甲酸铵（pH 3.5）和乙腈为流动相，采用Agilent Zorbax SB C₁₈（100 mm × 2.1 mm×3.5 μm）色谱柱进行分离，电喷雾离子源，正离子多反应监测扫描方式分析检测。除阿卡波糖和二甲双胍的检测限为20 mg•kg^-1外，其他降糖药的检测限均不高于1 mg•kg^-1，10种降糖药的回收率为75.2%~116.9%。

Abstract: Total of 10 antidiabetics illegally mixed into health products were simultaneously detected by LC-MS/MS. Methanol was used as the extraction solution. Mobile phase was included 10 mmol•L^-1 ammonium formate (pH 3.5) and acetonitrile. Agilent Zorbax SB C₁₈（100 mm × 2.1 mm×3.5 μm）column was used to separate the compounds, and mass data were acquired with the ESI(+) MRM mode. The LODs of acarbose and metformin are 20 mg•kg^-1, and the LODs of the other antidiabetics are no more than 1 mg•kg^-1. Recoveries of 10 drugs are 75.2%-116.9%.