Abstract: In the work, magnetic strong cation exchange(MSCX) resins were successfully prepared and applied to the extraction of melamine(MEL) from egg samples. The extraction procedure was carried out in a single step by blending and stirring the sample, extraction solvent and MSCX resins for 10 min. When the extraction was completed, the resins with adsorbed MEL were easily separated from the sample matrix by adscititious magnet field. Main factors affecting the extraction of MEL such as the amount of MSCX resins, extraction time, washing and eluting conditions were optimized. The MEL eluted from the resins was determined by liquid chromatography-tandem mass spectrometry. The recoveries of MEL are in the range of 77.2%-99.3%. The proposed method are successfully applied to the determination of MEL in eggs obtained from different local markets.