应用液相色谱-质谱联用技术同时测定花生中的黄曲霉毒素B1、B2、G1、G2

Simultaneous Determination of Aflatoxin B1, B2, G1, G2 in Peanut by LC-MS/MS

  • Abstract: The aflatoxin B1, B2, G1, G2 in peanut were develop by LC-MS/MS. The mass spectrometer was operated in the positive ion mode using multiple reaction monitoring. The sample was extracted with methanol, and chromatographic separation was carried out on a MGIII-C18 column(2.1 mm×150 mm×1.8 μm) with eluthic sion of V(acetonitrile):V(water)=35:65. The limit of detection is 0.5 μg•L-1, the limit of quantitation is
    0.5 μg•kg-1. The linear plots are between 0.5 μg•L-1 and 25.0 μg•L-1. Overall recoveries are between 88.3% and 99.2%, and RSD values are between 1.73% and 3.75%.

     

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