表面解吸常压化学电离质谱法直接测定饮料中的抗坏血酸

Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry for Direct Detection of Ascorbic Acid in Beverages

摘要: 建立了表面解吸常压化学电离质谱（SDAPCI-MS）测定饮料中抗坏血酸的方法。采用进样针取样，每次取样不多于5 nL，无需任何样品预处理，单个样品测定时间少于0.5 min。该方法对抗坏血酸的检出限可达1.9×10^-9g•mL^-1，线性范围为10^-8～10^-3 g•mL^-1，回收率为89.9 %～109.6 %，相对标准偏差（RSD）为3.2 %～5.8 %。

Abstract: A novel method was developed for direct detection of ascorbic acid in beverages by surface desorption atmospheric pressure chemical ionization mass spectrometry(SDAPCI-MS) without any sample pretreatment. Using sample needle sampling, the volume of each sampling was less than 5 nL. The average time for a single sample analysis was less than 0.5 min. The limit of detection for ascorbic acid is 1.9×10^-9 g•mL^-1, and the linear range is 10^-8—10^-3g•mL^-1. The recoveries for four samples spiked with 10^-4 g•mL^-1 ascorbic acid are in the range of 89.9%—109.6%, and the relative standard deviations in the range of 3.2%—5.8%.