Abstract: A simple and rapid method for the analysis of 12 bile acids was developed based on LC/MS. Quantification was finished in 7 min. For different bile acids, the detection limits ranged from 0.006 1 to 0.052 4 mg L^－1. The linear range was 2-3 magnitudes. The precisions of pretreatment for each bile acid were CVs＜10% except GCA. The developed method was applied to the analysis of human serums consisting of 22 cervical cancer women and 25 healthy women. The results revealed that GUDCA, GCDCA, TUDCA, TCDCA, TDCA, UDCA, CDCA and GCA were significantly increased in cervical cancer women.