Abstract: An analytical method for the determination of 25 kinds of organophosphate pesticide residues in medicinal herbs was established by gas chromatography-flame photometric detection (GC-FPD) and gas chromatography-ion trap mass spectrometry (GC-ITMS). The pesticide residues in samples were extracted with acetone by ultrasonic wave assistant extraction (UAE)， and purified by gel permeation chromatography(GPC). GC-ITMS was used as qualitative determination, whereas GC-FPD was used as quantify. This method can simultaneously analyze 25 kinds of organophosphate pesticide residues. When additive levels between 0.01 mg•kg^-1 and 0.04 mg•kg^-1, the range of recovery is between 66.58% and 111.20%，with RSD is between 0.75 % and 8.07%.