Abstract: A method for the simultaneous determination of 19 organochlorine pesticides(OCPs) in fish tissues was developed by gas chromatography-ion trap mass spectrometry(GC-IT-MS/MS). The samples were extracted by mixture of V (acetone)∶ V (hexane)= 1∶1 , then cleaned-up by gel permeation chromatography(GPC) and solid phase extraction(SPE), followed by analysis using GC-IT-MS/MS. The experimental results demonstrated good sensitivity for 19 target OCPs in spiked samples, by monitoring different precursor ions and product ions in electron ionization(EI) mode. Spiked reference samples are analyzed at concentration levels of 5.0 μg•kg^-1 and 10 μg•kg^-1, the average recoveries are range of 76.2%—106%, with a relative standard deviation(RSD) range of 4.8%—11%. Limits of detection(LOD) (S/N=3) are range of 0.03—1.42 μg•kg^-1. Limits of quantification(LOQ) (S/N=10) are between 0.1 μg•kg^-1 and 4.27 μg•kg^-1.