Abstract: A method for the determination of 10 mixed drugs of abuse in blood was developed by gas chromatography-mass spectrometry(GC/MS) coupled with accelerated solvent extraction(ASE). The target analytes were extraceted with V (ethyl acetate)∶V (cyclohexane)=1:1 by accelerated solvent extraction，and cleaned by auto gel permeation chromatography(GPC). The qualitative and quantitative analysis were carried out by GC/MS. The total ion chromatogram and selected ion chromatogram were obtained. The calibration of mdma, phenacetinum, ketamine, ramadol, cocaine, thebaine, papaverine, narcotine have a good linear relationship in the range of 1.2—7.2 mg•L^-1, meperidine has a good linear relationship in the range of 2.4—7.2 mg•L^-1, and herion has a good linear relationship in the range of 1.2—6 mg•L^-1. The linearity correlation cofficients of 10 mixed drugs of abuse are between 0.993 and 0.997. The average recoveries are 91%—97% with relative standard deviations of 2%—9.5%.