Abstract: The Application of Matrix-Assisted Laser Desorption Mass Spectrometry to BiochemistryZhao Shankai;Zhong Feng;Zhang Xiaohong(Instrumental Analysis & Research Center,Zhongshan University,Guangzhou 510275,China)Received Abstract:Matrix-assisted laser desorption mass spectrometry(MALDI-MS) has been used to determine the molecular weight of proteins with a home-made laser microprobe time offlight mass spectrometer.It demonstrated that this new method is possesed of high accuracy(±1 %~ 0.1%),high sensitivity(10-12~10-15mol) and rapidity(with few minutes),which are far better than those traditional methods.By the combination of SDS-PAGE and degradation,we had determined the molecular weight of proteins,polypeptides,conarschin protein and synthesized polymers with satisfied results.Keywords: matrix assisted laser desorption mass spectrometry,macromolecule,determination of molecular weight