Abstract: Solid-Liquid Phase Mixed Matrices Assisted Laser Desorption lonization Mass Spectrometry(MALDI-MS)Zhao Shankai;Zhu Zhihua(Instrumentation Analysis & Research Centre,Zhongshan University,Guangzhou 510275,China)Received Abstract:Solid-liquid binary mixture used for matrix-assisted laser desorption/ionization of peptides and proteins at 355 nm are described.By the use of mixed matrices composed of a solid-phase matrix(sinapinic acid)and a liquid-phase matrix(3-nitrobenzyl alcohol),the resolution,sensitivity and reproducibility of the obtained mass spectra can be improved.Keywords:matrix-assisted laser desorption/ionization,solid-liquid binary mixed matrices,protein,molecular weight determination