Abstract: Purity Control of Recombinant Human Epidermal Growth Factor by Matrix-Assisted Laser Desorption/Ionization Mass SpectrometryCat Yun;Liao Jie;Qian Xiaohong;Yang Songcheng; Dong Fangting;Zhou Jianzhong(National Biomedical Analysis Center,Beijing 100850,China)Wang Yongbo;Zhu Houchu(Institute of Biotechnology,Beijing 100071,China)Received Abstract:The molecular weight determination and mapping anlysis of recombinant hEGF by MALDI-TOF-MS are reported.81% of expected endoproteinase Gin-Cpeptides are detected.Alpha-Cyano-4-hydroxycinnamic acid is used as matrix.The purity of recombinant hEGF is detected by capillary electrophorsis.The purity is higher than 95%.Keywords:MALDI-TOF-MS,carillary electrophoresis,recombinant humant Epidermal Growth Factor(rhEGF)