Abstract: The headspace constituents of Artemisia argyi were sampled by single drop microextraction (SDME) and separated and identified by gas chromatography-mass spectrometry. Eucalytol(59.23%), camphor(5.26%), α-pinene(4.40%), camphene(3.64%), caryophyllene(3.23%), chrysanthenone(2.81%), cis-sabinene hydrate(2.08%), β-pinene(1.97%), borneol(1.91%), menthenol(1.85%), artemisia alcohol(1.57%), artemisia ketone(1.47%) are the most abundant volatiles released from Artemisia argyi, and 48 volatile compounds are identified.