Abstract: The essential oil from Vitis Vinifera L. seed was extracted by the simultaneous distillation extraction method. The chemical constituents of the volatiles from Vitis Vinifera L. seed were identified and determined by means of gas chromatography mass spectrometry (GC/MS). Thirteen compounds were identified. The main components are butylated hydroxytolene, 2 ethyl 1 hexanol, nonanal, α pinene, 2 pentyl furan, 3 carene, d limonene, etc. Percentages of the above compounds in the essential oil were obtained by peak area normalization method.