Abstract: The volatile oil was extracted from Stem of Acanthopanax sessilifours (Rupr.et Maxim.) seem. by using simultaneous distillation and extraction. The content of the volatile oil was 2.0%. Thirty eight peaks were separated and 23 compounds were identified by capillary gas chromatography mass spectrometry (GC/MS) with column HP 5 (30 m×0.25 mm×0.25 μL). The relative content of the components were determined with area normalization method. The main components are Farnesol (19.42%), Hexanoic acid ( 13.93 %), ( ) Spathulenol (13.10%), Butylated hydroxyloluene (6.41%), 3,7,11 Trimethyl 2,6,10 dodecatrien 1 ol acetate (5.73%) and Dibutyl phthalate (3.62%).