液相色谱/离子阱质谱法检测不同食用植物油中的叔丁基对苯二酚(TBHQ)

Quantification of TBHQ in Edible Vegetable Oil by Liquid Chromatography/Ion Trap Mass Spectrometry

摘要: 本文采用液相色谱/离子阱质谱法(LC/ITMS)对食用植物油中的抗氧化剂叔丁基对苯二酚(TBHQ)进行定量检测。样品经萃取后,以甲醇-水(体积比50∶50)作为流动相,以C18作为分离柱,以电喷雾离子源作为接口,在负离子检测模式下进行一、二级质谱分析。二级质谱检测的线性范围为61.8~4 542.5μg/L(R2=0.999 9),最低检出限为48μg/L;10种食用植物油中TBHQ的回收率为(81.9±1.9)%~(109.6±4.6)%,RSD(n=10)≤5.3%。该法能快速、简便、准确地检测食用植物油中的抗氧化剂TBHQ。

Abstract: Liquid chromatography/ion trap mass spectrometry(LC/ITMS) was used to quantify the synthetic phenolic antioxidant tertiary butylhydroquinone(TBHQ) in edible vegetable oil.After extracted,samples were analyzed under MS and MS/MS negative ion detection modes,with the mobile phase of methanol/water(50∶50,v∶v),a column of C_(18) and electrospray ionization interface.The liner calibration curves were obtained in the concentration range of 61.8-4 542.5 μg/L(R~2=0.999 9),the detection limit was 48 μg/L,and the recoveries of TBHQ from ten samples were(81.9±1.9)%-(109.6±4.6)%,RSD for TBHQ in edible vegetable oil was less than 5.3%(n=10).The method presented herein could quantify TBHQ in vegetable oil quickly,conveniently and accurately.